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1.
Rev. bras. anal. clin ; 52(1): 58-63, 20200330. tab
Article in Portuguese | LILACS | ID: biblio-1104474

ABSTRACT

Objetivo: Avaliar a ocorrência de anemia e presença de enteroparasitos relacionados a condições socioeconômicas e demográficas em idosos atendidos na Estratégia Saúde da Família (ESF) no município de Aparecida de Goiânia, Goiás, Brasil. Métodos: Trata-se de um estudo transversal, realizado com idosos atendidos na ESF do município de Aparecida de Goiânia. Foram coletadas fezes frescas e recém-eliminadas, as quais foram processadas pela técnica de Hoffman, Pons e Janer de 24 horas e coletadas amostras de sangue analisadas no aparelho automatizado Pentra 60®. Realizou-se análise estatística pelo programa BioEstat® versão 5.1 com medidas de associação odds ratio entre a presença de parasitos com a anemia, e as variáveis pesquisadas com o intervalo de confiança de 95% (IC 95%) e nível de significância de 5% (p < 0,05). Resultados: Foram avaliados os materiais biológicos de 101 idosos, e, destes, 15 (14,8%) apresentaram anemia. O estado civil, sexo, renda familiar, número de pessoas em casa, ocupação, consumo de frutas, verduras e legumes e hábitos comportamentais não apresentaram significância em relação à anemia. As variáveis com associação significativa (p<0,005) foram: nível baixo de escolaridade (p=0,05) e sedentarismo com consumo de álcool e/ou tabaco (p=0,05). Na análise das fezes encontrou-se positividade em uma amostra com presença de ovo de Ascaris lumbricoides. Conclusão: Os dados sobre a ocorrência de anemia podem variar bastante, dependendo da região e população estudada. O presente estudo demonstrou os fatores socioeconômicos e geográficos associados e baixa ocorrência de anemia e associação com parasitoses. A anemia não deve ser uma condição negligenciada durante o envelhecimento, portanto merece atenção e tratamento adequado.


Objective: To evaluate the occurrence of anemia and presence of enteroparasites related to socioeconomic and demographic conditions in the elderly attending the Family Health Strategy (FHS) in the municipality of Aparecida de Goiânia, Goiás, Brazil. Methods: This is a cross-sectional study, conducted with elderly people attended at the FHS of Aparecida de Goiânia. Fresh, newly disposed stools were collected and processed by the 24-hour Hoffman, Pons, and Janer technique and blood samples were collected on the Pentra 60® automated device. Statistical analysis was performed using the BioEstat® version 5.1 program, with odds ratios, between the presence of parasites with the anemia and variables surveyed with the 95% confidence interval (95% CI) and level. significance level of 5% (p <0.05). Results: The biological materials of 101 elderly individuals were evaluated, of which 15 (14.8%) presented anemia. Marital status, gender, family income, number of people at home, occupation, consumption of fruits, vegetables and behavioral habits were not significant in relation to anemia. The variables with significant association (p <0.005) were: low level of education (p = 0.05) and physical inactivity with alcohol and / or tobacco consumption (p = 0.05). In the analysis of feces was found positivity in one sample, presence of egg Ascaris lumbricoides. Conclusion: Data on the occurrence of anemia may vary widely, depending on the region and population studied. We demonstrated the associated socioeconomic and geographic factors and low occurrence of anemia and association with parasites. Anemia should not be a neglected condition during aging, so it deserves proper attention and treatment.


Subject(s)
Humans , Male , Female , Aged , Aged, 80 and over , Aged , Public Health , Anemia
2.
Rev. bras. anal. clin ; 51(1): 76-80, 30/03/2019. ilus, tab
Article in Portuguese | LILACS | ID: biblio-1008209

ABSTRACT

Objetivo: Validar o uso do teste sorológico para toxoplasmose em papel filtro em amostras de sangue de gestantes do município de Goiânia e região metropolitana. Métodos: Trata-se de um estudo prospectivo, onde foram coletadas 1.006 amostras de sangue em gestantes no município de Goiânia e região metropolitana. Todas as gestantes que concordaram participar da pesquisa assinaram um Termo de Consentimento Livre e Esclarecido. A avaliação do perfil sorológico foi realizada pela técnica de ELISA (ensaio imunoenzimático), com amostras de soro e papel filtro, para pesquisa de anticorpos anti-Toxoplasma gondii das classes IgM e IgG, sendo que o kit utilizado não é padronizado para a pesquisa de anticorpos IgM no papel filtro. A análise estatística foi processada no banco de dados do programa EpiInfo® versão 3.2.1, que avaliou a prevalência de soropositividade, frequência de positividade no soro, frequência de positividade no papel filtro, sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e índice kappa. Resultados: Na análise realizada no soro obtiveram-se 421 amostras positivas para anticorpos da classe IgG e três positivas para anticorpos da classe IgM. Em papel filtro obtiveram-se 443 positivas para anticorpos da classe IgG e uma positiva para anticorpos da classe IgM. Conclusão: O estudo permitiu identificar a semelhança de amostras positivas tanto com o uso do soro como no papel filtro para pesquisas de anticorpos da classe IgG, porém, para a pesquisa de anticorpos da classe IgM, o uso do soro apresentou maior sensibilidade quando comparado ao exame realizado com o papel filtro.


Objective: To validate the use of the serological test for toxoplasmosis in filter paper in blood samples from pregnant women from the city of Goiânia and the metropolitan region. Methods: This is a prospective study in which 1,006 blood samples were collected in pregnant women in the city of Goiânia and in the metropolitan region. All pregnant women who agreed to participate in the study signed a Free and Informed Consent Form. Serological profile evaluation was performed using ELISA (immunoenzymatic assay), with serum and filter paper samples, to investigate anti-Toxoplasma gondii antibodies of the IgM and IgG classes, and the kit used is not standardized for the research of IgM antibodies on the filter paper. Statistical analysis was performed in the database of the EpiInfo® version 3.2.1 program, which evaluated the prevalence of seropositivity, frequency of serum positivity, filter paper positivity frequency, sensitivity, specificity, positive predictive value, negative predictive value and index the kappa. Results: In the serum analysis, 421 samples were positive for antibodies of the IgG class and three were positive for antibodies of the IgM class. In the filter paper yielded 443 positive for antibodies of the IgG class and one positive for IgM class antibodies. Conclusion: The study allowed to identify the similarity of positive samples both with the use of serum and in the filter paper for IgG class antibodies. However, for serum IgM antibodies, the use of serum was more sensitive when compared to serum IgG. taken with the filter paper


Subject(s)
Humans , Female , Serology , Serologic Tests , Toxoplasmosis/diagnosis , Pregnant Women
3.
Rev. patol. trop ; 47(4): 207-216, dez. 2018. tab
Article in English | LILACS | ID: biblio-996624

ABSTRACT

Toxoplasmosis is an infection caused by the protozoan Toxoplasma gondii, whose definitive hosts are cats and whose intermediate hosts include humans.This research aims to evaluate aspects related to seroprevalence rates of anti-T. gondii antibodies among elderly users of the Primary Health Care Service in the municipality of Aparecida de Goiânia, State of Goiás, Brazil. A total of 101 elderly people participated in the study, and a questionnaire was applied to examine sociodemographic factors related to risk factors for toxoplasmosis. Five mL of peripheral blood were collected from all the participants for serological tests. IgM and IgG antibody screening was performed using ELISA. The average age of the participants was 69.5 years, the seroprevalence rate was 75.2% (76/101) of reactive IgG, and 24.8% (25/101) were non-reactive. IgM and IgG antibodies were found in 2.9% (3/101) of the participants. An IgG avidity test was performed, which revealed that the antibodies in the three samples were of low avidity, thus indicating that these individuals were in the acute phase of the infection. It was suggested that, in terms of socioeconomic conditions, an income of one to three monthly minimum salaries is the predominant risk factor for toxoplasmosis in this group.


Subject(s)
Humans , Aged , Aged, 80 and over , Seroepidemiologic Studies , Toxoplasmosis , Risk Factors
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